Inhibition of hcv replication in HCV replicon by shRNAs |
| |
| Authors: | Hamazaki Hiroyuki Takahashi Hitoshi Shimotohno Kunitada Miyano-Kurosaki Naoko Takaku Hiroshi |
| |
| Institution: | Department of Life and Environmental Sciences, Chiba Institute of Technology, Chiba, Japan. |
| |
| Abstract: | We show that the vector-derived long dsRNA specifically inhibits the replication of HCV RNA in HCV replicon. We designed a long dsRNA targeted to the full-length HCV IRES/core elements (1-to 377-nt). Our results revealed that the replication of HCV RNA was reduced to near background levels in a sequence-specific manner by the long dsRNAs in the HCV replicon. We also designed four shRNAs against several regions (120- to 139-nt, 260- to 279-nt, 330- to 349-nt, and 340- to 359-nt) of the HCV IRES/Core elements. The two HCV IRES/core-specific shRNAs, 330- to 349-nt and 340- to 359-nt, containing the AUG initiation codon sequence showed stronger HCV inhibitory effects than the other two shRNAs, 120- to 139-nt and 260- to 279-nt. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
