首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Indications for the enzymatic synthesis of 9-O-lactoyl-N-acetylneuraminic acid in equine liver
Authors:Reinhard G Kleineidam  Olaf Hofmann  Gerd Reuter  Roland Schauer
Institution:(1) Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstr. 40, W-2300 Kiel 1, Germany
Abstract:Fractionation of horse liver homogenate by centrifugation into heavy membranes at 10 000 × g, microsomal fraction at 105 000 × g, and the supernatant revealed sialate 9-O-lactoyltransferase activity only in the latter fraction. For the enzyme assay, the various fractions were incubated with14C labelled CMP-N-acetylneuraminic acid,N-acetylneuraminic acid and glycoconjugate-boundN-acetylneuraminic acid. Lactoylation was identified in three different TLC systems after acid hydrolysis and purification of the sialic acids in the incubation mixtures. Enzyme activity was found only in the supernatant fraction. Glycoconjugate-boundN-acetylneuraminic acid was the best substrate tested, although some lactoylation was also found when using CMP-N-acetylneuraminic acid.
Keywords:9-O-Lactoyl-N-acetylneuraminic acid  equine liver  enzymatic synthesis  sialic acid
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号