A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes |
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| Authors: | W A Loenen W J Brammar |
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| Affiliation: | Department of Biochemistry. University of Leicester, Adrian Building, Leicester LEI 7RH U.K. |
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| Abstract: | Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs (kb) generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII. Recombinants can be selected by their Spi- phenotype and their propagation is facilitated by the presence of a chi site. |
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| Keywords: | Recombinant DNA gene libraries Spi phenotype kb kilobase pairs PEG polyethylene glycol XG |
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