Morphological studies of the goldfish hindgut mucosa in organ culture |
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Authors: | Dr Hiroshi Iida Torao Yamamoto |
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Institution: | (1) Department of Anatomy, Kyushu University, Fukuoka, Japan;(2) Department of Anatomy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, 812 Fukuoka, Japan |
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Abstract: | Summary The morphology of the absorptive cells of the goldfish hindgut mucosa, and their capability for horseradish peroxidase (HRP) uptake, were investigated by electron microscopy after a 24-h organ culture. The columnar appearance and the fine structure of the absorptive cells were well preserved for 24 h at room temperature and 37° C with 5% CO2 in air, in all the media used in this study. Mitoses were frequently observed in the epithelium at the bottom of cultured mucosal folds, and re-epithelization was also observed in many explants.Some structural changes were, however, noted in the cultured absorptive cells, as compared with the non-cultured absorptive cells; the deep invaginations of the surface membrane between the microvilli decreased in number; supranuclear giant vacuoles were reduced in size or almost disappeared; the distributional pattern of mitochondria in the absorptive cells was altered.The HRP uptake experiments showed that the absorptive cells cultured for 24 h could still take up HRP by endocytosis and transport it, indicating that the absorptive cells maintained their capability of macromolecule uptake and transport after 24 h of culture. In addition, HRP experiments, in which reaction product was detected within numerous cytoplasmic tubules (CT), various vacuoles and CT-vacuole complexes, suggested a close relationship between CT and vacuolar system in the apical cytoplasm during endocytotic events in the absorptive cells. |
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Keywords: | Organ culture Intestinal absorptive cell (goldfish) Morphology Endocytosis Horseradish peroxidase |
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