发动蛋白诱导的囊泡内陷参与介导一氧化氮合酶对心肌收缩的调控

一氧化氮合酶(nitric oxide synthase,NOS)系统对正常或应激状态下心脏电-机械活动起着复杂的调控作用.本研究采用心肌细胞收缩与钙瞬变同步检测手段,研究NOS系统对心肌细胞收缩的潜在调控机制.在急性分离的正常大鼠心室肌细胞,100μmol/L spermine选择性抑制神经源性一氧化氮合酶(neur...

Dynamin-mediated endocytic process contributes to neuronal nitric oxide synthase-mediated regulation of cardiac contraction

Nitric oxide synthases (NOSs) play complex roles in the regulation of cardiac excitation contraction coupling under basal and stressed conditions. Herein, using the recording approach for intracellular calcium transient and synchronous myocyte contraction, the potential mechanism for NOSs-mediated cardiomyocyte contraction was explored. We found that selective inhibition of neuronal NOS (nNOS) with 100 μmol/L spermidine markedly enhanced the cardiomyocyte twitch [control: (10.5 ± 0.21)%; nNOS inhibition: (12.4 ± 0.18)%] and calcium transient [control: (0.27 ± 0.03)%; nNOS inhibition: (0.42 ± 0.01)%], but slowed the relengthening of twitch [control: (25.2 ± 1.3) ms; nNOS inhibition: (53 ± 2.8) ms] and the calcium transient decay [control: (129 ± 4.3) ms; nNOS inhibition: (176 ± 7.1) ms], which was similar to that by dynamin inhibition with 30 μmol/L dynasore. The nNOS inhibition- or dynasore-mediated effects could be rescued by an NO donor, S-Nitroso-N-acetylpenicillamine (SNAP). Our data suggest that the selective nNOS-mediated regulation of cardiac contractile activity may partly involve the dynamin-mediated endocytic mechanism.