| cAMP介导血清引起的嗅鞘细胞形态变化 |
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| 作者姓名： | Wang Y Huang ZH |
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| 作者单位： | 温州医学院检验医学院、生命科学学院;温州医学院低氧医学研究所基础医学院机能实验学教研室; |
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| 基金项目： | supported by Startup Foundation of Wenzhou Medical School (No. QTJ09013); Science Foundation of Educational Committee of Zhejiang Province,China (No. Y200906728); Wenzhou Science and Technology Bureau,China (No. H20100065) |
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| 摘 要： | 嗅鞘细胞是一类兼有星形胶质细胞和雪旺细胞特性的胶质细胞。培养的嗅鞘细胞存在两种能相互转化的形态亚型,然而转化的分子机制并不清楚。本研究旨在建立一种研究离体培养嗅鞘细胞形态转化的方法,基于该方法研究其相互转化的机制。采用原代培养大鼠嗅鞘细胞和免疫细胞化学技术,观察在有、无血清培养或给予双丁酰-环核苷酸(dB-cAMP)药物条件下嗅鞘细胞形态,并统计雪旺样和星形样嗅鞘细胞亚型的比例。结果显示:(1)在无血清培养条件下,(95.2±3.7)%嗅鞘细胞呈雪旺样形态,(4.8±3.7)%呈星形样形态;而在10%血清培养条件下,(42.5±10.4)%嗅鞘细胞呈雪旺样形态,(57.5±10.4)%呈星形样形态,随后换回无血清条件下培养24h,(94.8±5.0)%嗅鞘细胞呈雪旺样形态,(5.2±5.0)%呈星形样形态。(2)有无血清的培养条件并不影响嗅鞘细胞标记物p-75和S-100的表达。(3)在正常(10%)血清培养情况下,cAMP类似物dB-cAMP抑制F肌动蛋白应力纤维(F-actin stress fibers)和黏着斑(focal adhesion)形成,抑制血清引起的嗅鞘细胞形态变化,雪旺样细胞比例增加,并...
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| 关 键 词： | 嗅鞘细胞 形态可塑性 cAMP 血清 |
cAMP mediates the morphological change of cultured olfactory ensheathing cells induced by serum |
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| Wang Y,Huang ZH.cAMP mediates the morphological change of cultured olfactory ensheathing cells induced by serum[J].Acta Physiologica Sinica,2011,63(1):31-38. |
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| Authors: | Wang Ying Huang Zhi-Hui |
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| Institution: | WANG Ying2,HUANG Zhi-Hui1,1Institute of Hypoxia Medicine,Department of Function Experiments,2School of Laboratory Medicine and Life Science,Wenzhou Medical College,Wenzhou 325035,China |
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| Abstract: | Olfactory ensheathing cells(OECs) are a unique type of glia with common properties of astrocyte and Schwann cells.Cultured OECs have two morphological phenotypes,astrocyte-like OECs and Schwann cell-like OECs.Reversible changes have been found between these two morphological phenotypes.However,the molecular mechanism underlying the regulation of these reversible changes is still unknown.The aim of this paper is to establish a method for the morphology plasticity of cultured OECs,and investigate the underlyi... |
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| Keywords: | olfactory ensheathing cells morphology plasticity cAMP serum |
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