| 硫酸卡那霉素对成年大鼠的耳毒性效应 |
| |
| 作者姓名： | Zhang ZC Yu HM Liu Q Tian J Wang TF Lai CJ Zhou XY |
| |
| 作者单位： | 复旦大学附属眼耳鼻喉科医院耳鼻喉科;复旦大学脑科学研究院;复旦大学附属眼耳鼻喉科医院中心实验室; |
| |
| 基金项目： | supported by the Natural Science Foundation of Shanghai Science and Technology Committee,China(No.10ZR1406000); the Open Research Fund Program of the Institutes of Brain Science,Fudan University(2008-2010) |
| |
| 摘 要： | 本研究旨在观察硫酸卡那霉素(kanamycin sulfate,KM)对成年大鼠的耳毒性效应。6～7周龄的雄性Sprague-Dawley(SD)大鼠40只,随机分为2组:实验组,每天腹腔注射KM(500mg/kg)2周;对照组,注射等量生理盐水2周。通过检测脑干听觉诱发电位(auditory brainstem response,ABR)观察大鼠听力改变。ABR检测结束后,分离出耳蜗进行基底膜铺片、耳蜗冰冻切片,观察耳蜗螺旋神经节细胞(spiral ganglion cells,SGCs)的密度和耳蜗形态学改变。结果显示,注射KM2周后,大鼠在各频率的听觉阈值均有明显升高,其上升幅度超过60dB;随着时间推移,KM组SGCs密度逐渐降低,Corti器结构尚存,但外毛细胞及内毛细胞均有不同程度的缺失,以外毛细胞为甚;内毛细胞缺失与SGCs的密度下降相平行。以上结果表明,6～7周龄大鼠经过KM作用2周后,听力会明显下降,达到重度耳聋甚至全聋。KM的耳毒性作用与SGCs和内外毛细胞的损伤密切相关。
|
| 关 键 词： | 硫酸卡那霉素 耳聋 脑干听觉诱发电位 大鼠 |
Ototoxicity of kanamycin sulfate in adult rats and its underlying mechanisms |
| |
| Zhang ZC,Yu HM,Liu Q,Tian J,Wang TF,Lai CJ,Zhou XY.Ototoxicity of kanamycin sulfate in adult rats and its underlying mechanisms[J].Acta Physiologica Sinica,2011,63(2):171-176. |
| |
| Authors: | Zhang Zhi-Cun Yu Hong-Meng Liu Quan Tian Jie Wang Tian-Feng Lai Chui-Jin Zhou Xiao-Ya |
| |
| Institution: | ZHANG Zhi-Cun1,YU Hong-Meng1,3,LIU Quan1,TIAN Jie2,WANG Tian-Feng2,LAI Chui-Jin2,ZHOU Xiao-Ya11Department of Otolaryngology and 2Central Laboratory,Eye Ear Nose and Throat Hospital,Fudan University,Shanghai 200031,China,3The Institutes of Brain Science,Shanghai 200032 |
| |
| Abstract: | The aim of the present study was to assess the ototoxicity of kanamycin sulfate(KM) in adult rats and its underlying mech-anism.Forty male Sprague-Dawley rats(6-7 weeks old) were randomly divided into the experimental group and the control group.The animals in the experimental group were injected subcutaneously with KM(500 mg/kg per day) for two weeks,and the control group received equal volume of normal saline.To assess the ototoxicity of KM,the auditory brainstem response(ABR) was recorded to monitor the ... |
| |
| Keywords: | kanamycin sulfate hearing loss auditory brainstem response rat |
| 本文献已被 CNKI PubMed 等数据库收录！ |
|
