| 可溶性endoglin减弱人早孕细胞滋养细胞的浸润功能 |
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| 引用本文： | Dong W,Xu QX,Han YH,Yin LR. 可溶性endoglin减弱人早孕细胞滋养细胞的浸润功能[J]. 生理学报, 2011, 63(3): 267-271 |
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| 作者姓名： | Dong W Xu QX Han YH Yin LR |
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| 作者单位： | 天津医科大学第二医院产科; |
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| 基金项目： | supported by the Natural Science Foundation of Tianjin Municipality,China(No.09JCYBJC13000) |
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| 摘 要： | 本研究旨在探讨可溶性endoglin(soluble endoglin,sEng)对人早孕细胞滋养细胞活力和浸润功能的影响。采用胰蛋白酶-DNase消化法培养人早孕期(孕6～8周)细胞滋养细胞,传代后待细胞长满至70%～80%,分别加入没有添加(对照组)和添加sEng(10μg/L)的细胞培养液培养24h;Transwell技术检测细胞滋养细胞的浸润功能;RT-PCR法检测细胞滋养细胞基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(MMP-9)mRNA的表达;Western blot方法分别检测细胞滋养细胞MMP-2、MMP-9蛋白的表达。结果显示,sEng组细胞滋养细胞浸润能力低于对照组。与对照组比较,sEng组细胞滋养细胞MMP-2和MMP-9 mRNA及蛋白的表达明显下降(P<0.05)。以上结果提示,sEng可能通过调节人早孕细胞滋养细胞中MMP-2、MMP-9的表达而影响细胞的浸润能力,从而参与子痫前期的发生。
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| 关 键 词： | 子痫前期 细胞滋养细胞 基质金属蛋白酶 |
Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy |
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| Dong Wei,Xu Qun-Xing,Han Yu-Huan,Yin Li-Rong. Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy[J]. Acta Physiologica Sinica, 2011, 63(3): 267-271 |
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| Authors: | Dong Wei Xu Qun-Xing Han Yu-Huan Yin Li-Rong |
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| Affiliation: | DONG Wei,XU Qun-Xing,HAN Yu-Huan,YIN Li-Rong* Department of Obstetrics,the Second Hospital of Tianjin Medical University,Tianjin 300211,China |
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| Abstract: | |
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| Keywords: | preeclampsia cytotrophoblast matrix metalloproteinases |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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