Reactivation of chick erythrocyte nuclei in heterokaryons with rat hepatoma cells

The concentration of adenosine 3′,5′-cyclic monophosphate (cAMP) was measured in the parotid gland of the mouse after intraperitoneal injection of a beta-adrenergic drug, isoproterenol. A marked elevation of cAMP was observed 10 min after the administration, followed by a return to the control level within 40 min. A small peak was found around 14 h, onset of the stimulated DNA synthesis being observed at 20 h. A close relationship was found between the cAMP level at 10 min and the rate of DNA synthesis at 24 h in animals given different doses of the drug. However, DNA synthesis could not be induced by adrenalin in spite of a significant increase in the cAMP concentration. Furthermore, X-irradiation or certain metabolic inhibitors (actinomycin D and cycloheximide), administered prior to isoproterenol, completely inhibited the stimulated DNA synthesis without affecting the cAMP elevation at 10 min. It is concluded that the critical step in the initiation of stimulated DNA synthesis may be located at a period later than the initial cAMP elevation.