Suppression of experimental allergic encephalomyelitis (EAE) with liposome-encapsulated protease inhibitor: Therapy through the blood-brain barrier |
| |
Authors: | Taka Osanai Yoshitaka Nagai |
| |
Institution: | (1) Present address: Department of Biochemistry Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan |
| |
Abstract: | Experimental allergic encephalomyelitis (EAE) is an experimentally induced autoallergic demyelinating disease which is caused by immunization with a particular neuroantigen, such as myelin basic protein (MBP). Results have suggested that protease inhibitors might be useful therapeutically. Leupeptin (acetyl-l-leucyl-l-leucyl-argininal), a protease inhibitor of tripeptide nature, was effective in suppressing EAE in guinea pigs, when administered in a form of liposomes consisting of egg lecithin, cholesterol and sulfatide. The drug seemed to be transported into the central nervous system (CNS) tissues across the blood-brain barrier with the aid of a particular type of liposomes as vehicle. Some outbred Hartley guinea pigs completely recovered from distinct symptoms of EAE, such as loss of weight, paralysis, incontinence and/or diarrhea, when treated i.p. every day with lecithin-cholesterol-sulfatide (molar ratio, 4 5 1) reverse-phase evaporation vesicles-encapsulated leupeptin (REV-Leu) from day 6 after sesitization with 30 g of bovine MBP. Scarcely any typical histopathological changes of EAE were found in the CNS of most survivors treated with REV-Leu.Special Issue dedicated to Dr. Elizabeth Roboz-Einstein. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|