Mechanism of illegitimate recombination: common sites for recombination and cleavage mediated by E. coli DNA gyrase |
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| Authors: | H Ikeda I Kawasaki M Gellert |
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| Affiliation: | (1) Department of Molecular Biology, The Institute of Medical Science, The University of Tokyo, P.O. Takanawa, 108 Tokyo, Japan;(2) Laboratory of Molecular Biology, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, 20205 Bethesda, Maryland, USA |
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| Abstract: | Summary Illegitimate recombination dependent on DNA gyrase in a cell-free system has previously been described. We have now mapped DNA gyrase cleavage sites in the vicinity of known recombination sites in pBR322. Among five recombination sites examined, three were found to coincide with a DNA gyrase cleavage site. This result suggests that the cleavage of DNA by DNA gyrase has a central role in the recombination process. |
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