蒸汽爆破处理沙柳原料酶解条件优化

研究蒸汽爆破预处理对沙柳原料酶解效果的影响,通过响应曲面实验设计法优化蒸汽爆破处理沙柳原料的酶解工艺。结果表明,蒸汽爆破预处理沙柳原料的最佳蒸汽爆破处理条件：压力3.5 MPa、维压时间300 s; 蒸汽爆破最佳酶解条件：pH 4.8、温度53.5 ℃、 每克底物酶加量29.8 FPU。在最优条件下,蒸汽爆破处理沙柳原料的酶解率可以达到最大值87.92%,并验证了数学模型的有效性,试验结果表明蒸汽爆破预处理可以有效提高沙柳原料的水解率。

Optimization of enzymatic hydrolysis of steam explosion pretreated Salix psammophila

The steam explosion pretreatment was applied to Salix chips and the effects were evaluated by the enzymatic hydrolysis. Steam pretreated Salix enzymatic hydrolysis was optimized by a response surface methodology （RSM）. The results indicated that the best hydrolysis yields were attained when the steam explosion of the material was made at 3.5 MPa for 300 s. A Box-Behnken design of the RSM was employed to study the effects of temperature, enzyme concentration, and pH on pretreated Salix enzymatic hydrolysis. The optimum conditions for enzymatic hydrolysis were temperature 53.5 ℃, cellulase loading 29.8 FPU/g substrate, pH 4.8, respectively. A quadratic polynomial equation was obtained for enzymatic hydrolysis by multiple regression analysis using RSM. The experimental results were in agreement with that of the predicted model. Under the optimum conditions, the highest Salix hydrolysis yields were reached to 87.92%.