| 亚硫酸盐甘蔗渣浆酶解液发酵制备乙醇 |
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| 引用本文： | 余恒,刘宝田,欧阳嘉. 亚硫酸盐甘蔗渣浆酶解液发酵制备乙醇[J]. 生物加工过程, 2013, 0(5): 7-10 |
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| 作者姓名： | 余恒 刘宝田 欧阳嘉 |
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| 作者单位： | [1]南京林业大学化学工程学院,南京210037 [2]江苏省生物质绿色燃料与化学品重点实验室,南京210037 |
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| 基金项目： | 国家高技术发展研究计划（863计划）（2012AA022301）;教育部新世纪优秀人才支持计划（NCET-11-0988）;江苏省杰出青年基金（BK2012038）;江苏高校优势学科建设工程（PAPD） |
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| 摘 要： | 以亚硫酸盐甘蔗渣浆酶解液作为原料,利用C. shehatae发酵制取燃料乙醇。结果表明：还原糖最适初始质量浓度为葡萄糖140 g/L、木糖60 g/L、酶解液总糖80 g/L。利用初始葡萄糖55.06 g/L、木糖11.18 g/L、纤维二糖4.51 g/L的亚硫酸盐甘蔗渣浆酶解液发酵,经18 h获得乙醇22.98 g/L。乙醇得率为67.23%,葡萄糖利用率为99.27%,木糖利用率为32.96%,C. shehatae适合作为蔗渣为原料的乙醇发酵菌株。
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| 关 键 词： | 亚硫酸盐甘蔗浆 酶解液 休哈塔假丝酵母 燃料乙醇 |
Fuel ethanol fermentation using enzymatic hydrolysate of sulfite bagasse pulp |
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| YU Heng,LIU Baotian,OUYANG Jia. Fuel ethanol fermentation using enzymatic hydrolysate of sulfite bagasse pulp[J]. Chinese Journal of Bioprocess Engineering, 2013, 0(5): 7-10 |
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| Authors: | YU Heng LIU Baotian OUYANG Jia |
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| Affiliation: | 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Key Lab of Biomass-bassed Green Fuels and Chemicals, Nanjing 210037, China) |
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| Abstract: | Enzymatic hydrolysate of sulfite bagasse pulp was used as the substrate of the fermentation to product fuel ethanol. In order to achieve the highest sugar utilization ratio, the best initial concentration of reducing sugars were optimized as follows： glucose 140 g/L, xylose 60 g/L, and the total sugar of enzymatic hydrolysate 80 g/L. In the subsequent fermentation, the enzymatic hydrolysate containing glucose 55.06 g/L,xylose 11.18 g/L and cellobiose 4.51 g/L was used as the substrate, 22.98 g/L ethanol and 67. 23 % ethanol yield were obtained after 18 h fermentation. The glucose utilization rate and xylose utilization rate were 99.27% and 32.96%, respectively. C. shehatae could be used as appropriate strain by bagasse pulp into ethanol. |
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| Keywords: | sulfite pulp enzymatic hydrolysate C. shehatae fuel ethanol |
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