Identification of GroEL as a constituent of an mRNA-protection complex in Escherichia coli |
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| Authors: | Dimitris Georgellis,Bjö rn Sohlberg,F. Ulrich Hartl,Alexander von,Gabain |
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| Affiliation: | Institute of Microbiology and Genetics, Vienna Biocenter, Dr Bohr-Gasse 9, A-1030 Vienna, Austria.;Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden.;Howard Hughes Medical Institute in Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, N.Y. 10021, USA. |
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| Abstract: | An RNA-binding activity has been identified in Escherichia coli that provides physical protection of RNA against ribonucleases in an ATP- and Mg2+-dependent manner. This binding activity is stimulated under growth conditions known to cause a decrease in the rate of mRNA decay. RNA protection is mediated by a protein complex that contains a modified form of the chaperonin GroEL as an indispensable constituent. These results suggest a new role for GroEL as an RNA chaperone. |
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