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A protein microarray prepared with phage-displayed antibody clones |
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| Authors: | Bi Qun Cen Xiaodong Wang Wenjuan Zhao Xinsheng Wang Xuan Shen Ti Zhu Shenggeng |
| Affiliation: | aCollege of Life Sciences, Peking University, Beijing 100871, PR China bState Key Laboratory of Molecular Dynamic and Stable Structures, Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, PR China cNational Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, PR China dPeking Union Medical College Hospital, Beijing 100730, PR China |
| Abstract: | Using a large phage antibody library, a protein microarray spotted directly with phage-displayed antibody clones was created to discriminate between recognition profiles of samples from healthy donors and leukemia patients. The protocol for preparing antibody-displaying phage chips was presented. Some conditions such as substrates and blocking buffers were compared and optimized. The major improvements of this microarray are higher throughput and lower cost compared to previous antibody chips. Due to its convenience and sensitivity, it can be extensively used for rapid and high throughput detection of protein profiles of experimental and clinical samples. |
| Keywords: | Phage display Microarray Antibody library pIII system pVIII system |
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