Growth of a methanol-utilizing yeast |
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Authors: | HS Pal IY Hamdan |
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Institution: | Kuwait Institute for Scientific Research, P.O. Box 24885, Kuwait |
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Abstract: | A halophilic thermotolerant yeast species, identified as Hansenula polymorpha Morais et Maia, was isolated from a mixed culture obtained from sea-water from the Arabian Gulf. The species grew on methanol at 25–42°C, pH 3.5–6.7, and in a medium compounded with 75% sea-water. Either thiamin HCl and biotin or yeast extract proved essential for growth. In shake flask studies a depression of the yield was observed when methanol concentration increased; at concentrations in excess of 0.1%, v/v, inhibition of growth also occurred. In a batch culture grown in a 14 l fermenter, the values of Td, μ and Ys were found to be 3 h, 0.23 h?1 and 0.38, respectively. |
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