Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean |
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| Authors: | Rui Guo Ming Ding Si-Liang Zhang Gen-jun Xu Fu-kun Zhao |
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| Institution: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;(2) Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai, 20031, China;(3) College of Life Science, Zhejiang Sci-Tech University, Hongzhou, 310018, China;(4) Room 543, 320 Yue-yang Road, Shanghai, 200031, China |
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| Abstract: | Cellulase genes have been reported not only from fungi, bacteria and plant, but also from some invertebrate animals. Here,
two cellulase (endo-β-1,4-glucanase, EC 3.2.1.4) genes, eg27I and eg27II, were cloned from the freshwater snail Ampullaria crossean cDNA using degenerate primers. The nucleotide sequences of the two genes shared 94.5% identity. The open reading frames of
both genes consisted of 588 bp, encoding 195 amino acids. Both EG27I and EG27II belong to the glycoside hydrolase family 45,
and each lacks a carbohydrate-binding module. The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and,
in addition, successful cloning of EG27 cDNA supported endogenous production of EG27 cellulase by Ampullaria crossean. Investigation of the EG27 cDNA from A. crossean will provide further information on GHF45 cellulases. |
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| Keywords: | Cellulase Endoglucanase EG27 Ampullaria crossean Gene cloning |
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