| 人Ⅱ型囊泡单胺转运体基因的克隆 |
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| 引用本文： | 张京钟,余爽,段德义,苏玉金,杨慧,徐群渊.人Ⅱ型囊泡单胺转运体基因的克隆[J].中国组织化学与细胞化学杂志,2002,11(3):259-263. |
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| 作者姓名： | 张京钟 余爽 段德义 苏玉金 杨慧 徐群渊 |
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| 作者单位： | 首都医科大学北京神经科学研究所,北京市神经再生修复重点实验室,北京100054 |
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| 基金项目： | 国家重点基础研究计划 [No .G19990 5 4 0 0 8]，北京市青年骨干教师基金资助 |
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| 摘 要： | 目的 为获得人Ⅱ型囊泡单胞转运体（vesicular monomine transporter-2VMAT2)以治疗帕金森病，克隆添加Kozak序列的VMAT2cDNA，用于真核细胞表达。方法 从人胎脑中提取总RNA，逆转录成cDNA，设计一对引物用PCR方法扩增目的cDNA片段并将其重级一起pGEM-Easy-T载体中，酶切鉴定插入片段后，进行全序列测定。结果 RT-PCR扩增到1637bp的带有Kozak序列的cDNA片段。结论 克隆人VMAT2cDNA片断，可用于真核细胞表达。
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| 关 键 词： | 基因克隆 帕金森病 人Ⅱ型囊泡 单胞转运体 |
| 修稿时间： | 2002年2月1日 |
MOLECULAR CLONING OF VESICULAR MONOAMINE TRANSPORTER-2 cDNA FROM HUMAN FETAL BRAIN |
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| Zhang Jingzhong,Yu Shuang,Duan Deyi,Su Yujin,Yang Hui,Xu Qunyuan.MOLECULAR CLONING OF VESICULAR MONOAMINE TRANSPORTER-2 cDNA FROM HUMAN FETAL BRAIN[J].Chinese Journal of Histochemistry and Cytochemistry,2002,11(3):259-263. |
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| Authors: | Zhang Jingzhong Yu Shuang Duan Deyi Su Yujin Yang Hui Xu Qunyuan |
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| Abstract: | Objective To obtainthe Chinese human geneof vesicular monoamine transporter 2 (VMAT2) for the treatment of Parkinson's disease Method Total cellular RNA was isolated from human fetal brain and reversely transcribed into cDNA The PCR products were inserted into the pGEM Easy T vector for sequencing after an analysis of the recombinant pGEM Easy T plasmids by restriction enzymes Result The sequence of the target DNA fragment was 1637bp, which matched the size of purpose Conclusion The resultsshow that the full length cDNA of Chinese VMAT2 with additional Kozak sequence was cloned and could be expressed in eukaryotic cells |
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| Keywords: | VMAT2 Gene Clone Parkinson's Disease Human |
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