Characterization of a factor produced by human alloreactive T lymphocyte clones, active on the eosinophils and precursors of erythroid strains

Alloreactive T-cell clones obtained from cells infiltrating a human rejected kidney graft were shown to produce upon specific antigenic stimulation with interleukin 2, a factor triggering the proliferation of an interleukin 3 dependent murine cell line. The lymphokine responsible for this activity was a monomeric glycoprotein (MW = 41,000) exhibiting on one hand chemotactic as well as activating properties on murine and human eosinophils respectively, and on the other hand a burst promoting activity. Its biochemical and biological features strongly suggest that this factor cannot be likened to any known human lymphokine.