Biochemical systematics of the genus Lipara using isoenzymes

ln the course of a systematic and population genetic study of the genus Lipara, a new approach was tested, involving the examination of isoenzymes using PAGE and isoelectric focusing (IEF). The enzymes from whole individual specimens of both larvae or adult flies were analysed. Twenty-five enzyme systems have been investigated with PAGE, 18 of which gave interpretable results. General proteins were analysed with both PAGE and IEF. By means of these techniques, the intra- and interspecific variation of structural proteins and different enzyme systems were examined. Possible changes in general proteins and enzymes during the development from larvae to adults were also investigated.