Enhanced biocatalytic production of <Emphasis Type="SmallCaps">l</Emphasis>-cysteine by <Emphasis Type="Italic">Pseudomonas</Emphasis> sp. B-3 with in situ product removal using ion-exchange resin |
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Authors: | Email author" target="_blank">Pu?WangEmail author Email author" target="_blank">Jun-Yao?HeEmail author Jiang-Feng?Yin |
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Institution: | 1.College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou,People’s Republic of China;2.Institute of Biopharmacy,Zhejiang Pharmaceutical College,Ningbo,People’s Republic of China |
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Abstract: | Bioconversion of dl-2-amino-Δ2-thiazoline-4-carboxylic acid (dl-ATC) catalyzed by whole cells of Pseudomonas sp. was successfully applied for the production of l-cysteine. It was found, however, like most whole-cell biocatalytic processes, the accumulated l-cysteine produced obvious inhibition to the activity of biocatalyst and reduced the yield. To improve l-cysteine productivity, an anion exchange-based in situ product removal (ISPR) approach was developed. Several anion-exchange resins were tested to select a suitable adsorbent used in the bioconversion of dl-ATC for the in situ removal of l-cysteine. The strong basic anion-exchange resin 201 × 7 exhibited the highest adsorption capacity for l-cysteine and low adsorption for dl-ATC, which is a favorable option. With in situ addition of 60 g L?1 resin 201 × 7, the product inhibition can be reduced significantly and 200 mmol L?1 of dl-ATC was converted to l-cysteine with 90.4 % of yield and 28.6 mmol L?1 h?1 of volumetric productivity. Compared to the bioconversion without the addition of resin, the volumetric productivity of l-cysteine was improved by 2.27-fold using ISPR method. |
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