|
|||||
|
|
Trehalose lipid biosurfactants produced by the actinomycetes |
|
| Authors: | Johannes?H.?Kügler author-information" > author-information__contact u-icon-before" > mailto:johannes.kuegler@kit.edu" title=" johannes.kuegler@kit.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Claudia?Muhle-Goll,Boris?Kühl,Axel?Kraft,Raphael?Heinzler,Frank?Kirschh?fer,Marius?Henkel,Victor?Wray,Burkhard?Luy,Gerald?Brenner-Weiss,Siegmund?Lang,Christoph?Syldatk,Rudolf?Hausmann |
| Affiliation: | 1.Institute of Process Engineering in Life Sciences, Section II: Technical Biology,Karlsruhe Institute of Technology (KIT),Karlsruhe,Germany;2.Institute of Organic Chemistry,Karlsruhe Institute of Technology (KIT),Karlsruhe,Germany;3.Institute of Functional Interfaces, Department Microbiology of Natural and Technical Interfaces,Karlsruhe Institute of Technology (KIT),Eggenstein-Leopoldshafen,Germany;4.Department of Molecular Structural Biology,Helmholtz Centre for Infection Research,Braunschweig,Germany;5.Institute for Biological Interfaces,Karlsruhe Institute of Technology (KIT),Eggenstein-Leopoldshafen,Germany;6.Institute for Biochemistry, Biotechnology and Bioinformatics, Department of Biotechnology,Technical University Braunschweig,Braunschweig,Germany;7.Institute of Food Science and Biotechnology, Section Bioprocess Engineering,University of Hohenheim,Stuttgart,Germany |
| Abstract: | Actinomycetales are known to produce various secondary metabolites including products with surface-active and emulsifying properties known as biosurfactants. In this study, the nonpathogenic actinomycetes Tsukamurella spumae and Tsukamurella pseudospumae are described as producers of extracellular trehalose lipid biosurfactants when grown on sunflower oil or its main component glyceryltrioleate. Crude extracts of the trehalose lipids were purified using silica gel chromatography. The structure of the two trehalose lipid components (TL A and TL B) was elucidated using a combination of matrix-assisted laser desorption/ionization time-of-flight/time-of-flight/tandem mass spectroscopy (MALDI-ToF-ToF/MS/MS) and multidimensional NMR experiments. The biosurfactants were identified as 1-α-glucopyranosyl-1-α-glucopyranosid carrying two acyl chains varying of C4 to C6 and C16 to C18 at the 2′ and 3′ carbon atom of one sugar unit. The trehalose lipids produced demonstrate surface-active behavior and emulsifying capacity. Classified as risk group 1 organisms, T. spumae and T. pseudospumae hold potential for the production of environmentally friendly surfactants. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |