A new approach to the depletion of albumin and immunoglobulin G from human serum |
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| Authors: | E?A?Bormotova B?L?Mil’man Email author" target="_blank">T?V?GupalovaEmail author |
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| Institution: | 1.Institute of Experimental Medicine, Northwest Branch,Russian Academy of Medical Sciences,St. Petersburg,Russia |
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| Abstract: | The use of proteomic analysis to find potential diagnostic biomarkers is limited by the presence of serum albumin (HSA) and immunoglobulin (IgG) at high concentrations in patients’ blood; these substances impede the detection of serum proteins with similar molecular weights. Recombinant HSA- and IgG-binding polypeptides are used as ligands in creating sorbents for complete removal of the proteins by affinity chromatography. The binding specificity of the sorbents for HSA and IgG is higher than that of the conventionally used antibodies. A composite sorbent enabling the depletion of HSA and IgG from serum by single-step affinity chromatography was obtained. The developed sorbents were used to prepare serum for proteomic analysis. |
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