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Characteristic of Polysaccharide Complexes from <Emphasis Type="Italic">Centaurea scabiosa</Emphasis> L. and <Emphasis Type="Italic">Centaurea pseudomaculosa</Emphasis> Dobrocz.
Authors:Email author" target="_blank">S?LarkinaEmail author  S?V?Krivoshchekov  A?M?Guryev  T?V?Kadyrova  E?V?Ermilova  V?V?Kotserubskaya  M?S?Yusubov
Institution:1.Siberian State Medical University,Tomsk,Russia;2.Tomsk Polytechnic University,Tomsk,Russia
Abstract:We characterized polysaccharide complexes from Centaurea scabiosa L. and Centaurea pseudomaculosа Dobrocz. We proposed the technique of sequential selection of water-soluble polysaccharides and pectin substances from the aerial parts of studied objects. We have discovered that the content of water-soluble polysaccharides in the aerial parts of C. scabiosa was 2.8 times higher (2.7 ± 0.3%, n = 3) than in C. pseudomaculosа (0.97 ± 0.50%, n = 3). The content of pectin substances in the aerial parts of C. scabiosa was 2 times higher (7.6 ± 0.4%, n = 3) than in C. pseudomaculosа (3.9 ± 0.3%, n = 3). The residues of D-galacturonic acid, L-rhamnose, D-xylose, D-mannose, D-glucose, and D-galactose are the monomeric units of polysaccharide complexes from C. scabiosa and C. pseudomaculosa. Using ion-exchange chromatography, three polysaccharide fractions (molecular weights 667, 722, and 1027 kDa), whose monomer units are D-galacturonic acid, L-rhamnose, D-galactose, D-xylose, and D-glucose were isolated from the water-soluble polysaccharides of C. scabiosa.
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