Cellular compartmentation of calcium in mouse fibroblasts in vitro depends on cell density and transformation |
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| Authors: | T Hartmann K Seuwen G Adam |
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| Affiliation: | 1. Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA;2. Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA;3. PEGASE, INRAE, Institut Agro, 35590 Saint-Gilles, France;4. Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA;1. School of Environment and Resources, Taiyuan University of Science and Technology, Taiyuan 030024, Shanxi Province, China;2. State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;3. Engineering Research Center of Coal-based Ecological Carbon Sequestration Technology of the Ministry of Education, Shanxi Datong University, 037009, China |
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| Abstract: | Cellular compartmentation of Ca has been investigated by kinetic analysis of 45Ca efflux from preloaded cells at various states of cell density-dependent proliferation of normal (3T3) and transformed (3T6 and SV40-3T3) mouse cells. Three pools of exchangeable calcium were separated on the basis of their differing exchange kinetics. For each of the cell lines tested, all three compartments decrease with cell density. Significant differences between normal and transformed cells are observed upon quiescence of the normal cells, where the slowly exchanging compartment of normal cells gradually increases, whereas that of the transformed cells continues to decrease (with increasing cell density). Free cytoplasmic Ca2+ concentration as determined by the Quin 2 method, was found to be significantly higher in transformed cells than in normal cells. These results indicate significant differences in Ca homeostasis between normal and transformed cells. |
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