| 缺氧对肺动脉内皮细胞MMP-2、MMP-9表达的影响 |
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| 引用本文： | 叶红,马万里,曹福元,柯丹,刘声远,王迪浔.缺氧对肺动脉内皮细胞MMP-2、MMP-9表达的影响[J].中国组织化学与细胞化学杂志,2005,14(5):511-514. |
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| 作者姓名： | 叶红 马万里 曹福元 柯丹 刘声远 王迪浔 |
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| 作者单位： | 1. 华中科技大学同济医学院病理生理系,卫生部呼吸系疾病重点实验室,武汉,430030
2. 华中科技大学同济医学院附属协和医院呼吸内科,武汉,430030 |
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| 摘 要： | 目的观察缺氧对培养的猪肺动脉内皮细胞基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的表达的影响.方法采用RT-PCR、Western blot、底物胶电泳(酶谱图)、免疫细胞化学等方法.结果肺动脉内皮细胞缺氧24h可使MMP-2的mRNA表达和蛋白分泌减少,酶活性减弱,与常氧组比较有显著性差异, MMP-9无明显变化.结论缺氧时MMP-2降低,溶解细胞外基质的能力降低,可能是缺氧性肺血管构型重组的机制之一.
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| 关 键 词： | 缺氧 肺动脉内皮细胞 基质金属蛋白酶 |
| 收稿时间： | 11 15 2004 12:00AM |
| 修稿时间： | 01 21 2005 12:00AM |
EFFECT OF HYPOXIA ON MMP-2 AND MMP-9 EXPRESSION IN PULMONARY ARTERY ENDOTHELIAL CELLS |
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| Ye Hong,Ma Wan-li,Cao Fuyuan,Ke Dan,Liu Shengyuang,Wang Dixun.EFFECT OF HYPOXIA ON MMP-2 AND MMP-9 EXPRESSION IN PULMONARY ARTERY ENDOTHELIAL CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2005,14(5):511-514. |
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| Authors: | Ye Hong Ma Wan-li Cao Fuyuan Ke Dan Liu Shengyuang Wang Dixun |
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| Institution: | Pulmonary Laboratory of Ministry of Public Health of China, Department of Pathoph ysiology , 1 Respiratory Department of Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 , China |
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| Abstract: | Objective To investigate the effect of hypoxia on the expression of MMP-2 and MMP- 9 in pulmonary arterial endothelial cells. Methods RT-PCR, gelatin zymography, western blot, and immunocytochemistry were used. Results After exposure to hypoxia for 24h, the expression of MMP-2 mRNA and protein, as well as the activity, decreased significantly. However, MMP-9 expression was not affected by hypoxia. Conclusion Hypoxia can induce the reduction of MMP-2 expression, which may lead to a decrease in matrix-degradation and contribute to the mechanism of hypoxic pulmonary vascular remodeling. |
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| Keywords: | Hypoxia Pulmonary arterial endothelial cell MMPs |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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