Identification of two forms of PFK and a fructose-2,6-bisphosphate independent form of PFP in a green alga |
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| Authors: | F Kiss T C Johnson A L Klecan Gy Vincze B B Buchanan A Balogh |
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| Institution: | (1) Department of Biology and Chemistry, György Bessenyei College, Nyiregyháza, Hungary;(2) Division of Molecular Plant Biology, University of California, 94702 Berkeley, California, USA;(3) Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI |
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| Abstract: | Cell-free preparations from the green alga, Chlorella pyrenoidosa, contained two forms of phosphofructokinase (PFK), designated PFK I and PFK II. This represents the first evidence for a second form of PFK in green algae. A pyrophosphate D-fructose-6-phosphate, 1-phosphotransferase (PFP) activity, that was unaffected by the regulatory metabolite, fructose-2,6-bisphosphate, co-purified with PFK II through several steps. The data suggest that Chlorella pyrenoidosa resembles higher plants in containing two forms of PFK, but differs in containing an atypical form of PFP.Abbreviations PFK
phosphofructokinase
- PFP
pyrophosphate D-fructose-6-phosphate, 1-phosphotransferase, Fru-2,6-P2-fructose-2,6-bisphosphate
- DEAE
diethylaminoethyl- |
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| Keywords: | fructose-2 6-bisphosphate PFK PFP Chlorella green algae carbon metabolism |
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