Effect of Plant AntifreezeProteins on Porcine Embryo to Cryopreservaton |
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Authors: | FEI Yun-biao WEI Ling-bo GAO su-qin ZHAO Shu-hui JIANG Yong ZHU Hua-bin LUO Ying-rong ZHANG Zhi-xuan |
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Abstract: | This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The developmental rates of porcine embryos frozen with 0.5 mg/ml AFPs in freeze medium and cultured for 12 and 24 hours in vitro are 25% and 0%respectively. With the concentration of AFPs increased to 5 mg/ml, the corresponding values became 80% and 40%. The hatched rates for porcine embryos frozen with 0.5 mg/ml and 5 mg/ml of AFPs and cultured for 24 hours in vitro are 0% and 20% respectively..The developmental and hatched rate of the contnrol are all 0%(0/4). These results indicate that the survival rates of porcine expanded and hatched blastocyst can be improved by supplementing freeze medium with plant AFPs. |
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Keywords: | Porcine embryo Cryopreservation Plant antifreeze protein |
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