Sucrose-binding lectin in regenerating cockroach (Periplaneta americana) legs: Its purification from adult hemolymph |
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| Affiliation: | 1. Division of Pediatric Cardiology, University of Utah Department of Pediatrics, Salt Lake City, UT 84113, USA;2. Division of Anatomic Pathology, University of Utah Department of Pathology, Salt Lake City, UT 84113, USA;1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;2. Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China;1. Soonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Cheonan 31151, Republic of Korea;2. Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, Republic of Korea |
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| Abstract: | Previously, we purified Periplaneta lectin from the hemolymph of adult Periplaneta americana (American cockroach) (Kubo and Natori Eur. J. Biochem. 168, 75–82, 1987). Immunoblotting analysis using antibody against Periplaneta lectin showed that the cockroach hemolymph contains another lectin that cross reacted immunologically with Periplaneta lectin. We have purified this new lectin (regenectin) to homogeneity. Affinity purified antibody against regenectin cross reacted with Periplaneta lectin. Thus, Periplaneta lectin and this new lectin were found to be different lectins sharing common antigenicity. After leg amputation, this new lectin was found to appear transiently at a specific stage of regeneration as revealed by immunoblotting, suggesting that it plays a role in the regeneration process. |
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