Inhibition of the accumulation of viral polypeptides in the densonucleosis virus-infected midgut of the silkworm,Bombyx mori,reared at a supraoptimal temperature |
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| Institution: | 1. The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK;2. AgroEcology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK;3. Department of Agroecology, Aarhus University, Flakkebjerg Research Centre, Forsoegsvej 1, DK-4200 Slagelse, Denmark;4. INRA, UR 1115, PSH, Plantes et Systèmes de Culture Horticoles, 84000 Avignon, France;5. J.K. Pell Consulting, Wardown Crescent, Bedfordshire, Luton LU2 7JT, UK;6. Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France;7. Bio-Protection Research Centre, Burns, 514, Cnr Springs and Ellesmere Junction Roads, P O Box 85084, Lincoln University, Lincoln 7647, New Zealand;1. Research Division Agroecology and Environment, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland;2. USDA-ARS, Arid-Land Agricultural Research Center, 21881 N. Cardon Lane, Maricopa, AZ 85138, USA;3. Department of Entomology, Cornell University, 630 W. North St., New York State Agricultural Experiment Station, Geneva, NY 14456, USA;1. Biosystematics Group, Wageningen University, Wageningen, the Netherlands;2. Education and Student Affairs, Wageningen University, Wageningen, the Netherlands;3. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, the Netherlands;4. Department of Agricultural, Food and Forest Sciences, University of Palermo, Palermo, Italy;1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Department of Entomology, National Chung Hsing University, Taichung City 402, Taiwan;2. Department of Plant Medicine, National Pingtung University of Science & Technology, Pintung 912, Taiwan;3. Taichung District Agricultural Research and Extension Station, COA, Changhua 515, Taiwan;4. Department of Agricultural Biology, Jeonbuk National University, Jeonju 54596, Korea;5. Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54596, Korea |
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| Abstract: | Effect of a supraoptimal temperature on the accumulation of viral polypeptides in the midgut was examined by immunoblot analysis in the larvae of the silkworm, Bombyx mori, infected with Bombyx densonucleosis virus type 2. In the larvae reared continuously at 25°C, viral polypeptides were first detected in the midgut at 2 days postinfection (pi) and in the feces at 4 days pi. When the larvae inoculated per os with the virus for 24 hr at 25°C were immediately shifted to 35°C, there were no detectable viral polypeptides in both the midgut and feces throughout the experiment. In the infected larvae shifted from 25° to 35°C at 48 hr pi, viral polypeptides preexisting in the midgut decreased to an undetectable level within 48 hr after the temperature shift, and no viral polypeptides were detected thereafter. Viral polypeptides in the feces of these larvae became detectable at 48 hr (4 days pi) after the temperature shift, as in the larvae at 25°C, and disappeared by 96 hr (6 days pi). These results indicate that a supraoptimal temperature inhibits accumulation of viral polypeptides in the midgut. It is likely that inhibited production of viral polypeptides rather than enhanced discharge of the infected midgut cells is responsible for the inhibited accumulation of viral polypeptides in the midgut at 35°C. |
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