Juvenile hormone esterase is a biochemical anti-juvenile hormone agent |
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| Institution: | 1. Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto Nacional de Tecnología Agropecuaria EEA Rafaela, Ruta 34 Km 227, Rafaela, 2300, Santa Fe Province, Argentina;2. Instituto Nacional de Tecnología Agropecuaria EEA Rafaela, Ruta 34 Km 227, Rafaela, 2300, Santa Fe Province, Argentina;3. Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Los Reseros y Las Cabañas, Castelar, 1712, Buenos Aires, Argentina;1. School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada;2. Departamento de Medicina y Zootecnia en Abejas, FMVZ, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Mexico, D.F. 04960, Mexico;3. Department of Mathematics and Statistics, University of Guelph, Guelph, ON N1G 2W1, Canada;1. Department of Ecology and Evolution, Biophore, University of Lausanne, 1015 Lausanne, Switzerland;2. Fasteris SA, 1228 Plan-les-Ouates, Switzerland |
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| Abstract: | Juvenile hormone esterase, purified by affinity chromatography from the larval hemolymph of Manduca sexta in the fifth stadium, was injected into larvae of the same species in the earlier stadia resulting in a blackening of the cuticle following ecdysis to the next larval stadium. This anti-juvenile hormone response was dose-dependent for an injection in the second, third or fourth stadium. Cuticular blackening was prevented by treating larvae with the juvenoid epofenonane. Larval response to injected juvenile hormone esterase also varied with the time of injection within a single stadium, having a maximum effect for injections at the time of head capsule slippage. Juvenile hormone esterase activity measured from the hemolymph after injection of larvae in the second stadium decreased over an 11 h time-course. Because the anti-juvenile hormone effects resulting from a single injection of juvenile hormone esterase were dependent on the time of injection, it appears that when juvenile hormone biosynthesis is active in the insect, the duration of enzyme activity limits the anti-juvenile effects that can be induced. |
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