Development and application of a radioimmunoassay for the juvenile hormones |
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| Affiliation: | 1. Department of Entomology, University of Wisconsin-Madison, WI 53706, USA;2. Sandoz Crop Protection Corp., 975 California Avenue, Palo Alto, CA 94304 USA;3. Department of Biology, University of Notre Dame, Notre Dame, IN 46556, U.S.A.;4. School of Chemical Science, Universiti Sains Malaysia, 1800 USM, Penang, Malaysia;1. Eskisehir Technical University, Faculty of Science, Department of Biology, TR26470, Eskisehir, Turkey;2. Eskisehir Technical University, Department of Materials Science and Engineering, TR26480, Eskisehir, Turkey;3. Anadolu University, Graduate School of Sciences, Department of Materials Science and Engineering, TR26480, Eskisehir, Turkey;4. Entekno Materials, Ltd, Anadolu Teknopark, No: 104 Yunus Emre Campus, TR26470, Eskisehir, Turkey;1. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada;2. Department of Natural History, Royal Ontario Museum, 100 Queens Park, Toronto, Ontario M5S 2C6, Canada;3. Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, USA;4. Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA;5. Department of Biological Sciences, Boise State University, 1910 University Dr., Boise, ID 83725-1515, USA;1. Department of Food Engineering, Faculty of Agriculture, Selcuk University, Konya, Turkey;2. Department of Food Engineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, Konya, Turkey;3. Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey;4. Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, İstanbul, Turkey;1. Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, College of Biotechnology, University of Santiago de Compostela, Rúa Carballo Calero s/n, Campus Lugo, E-27002, Lugo, Spain;2. Food Control Department, Faculty of Veterinary Medicine, Zagazig University, 44519, El Sharkia, Egypt;3. Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, 44519, El Sharkia, Egypt;1. Department of Biosystems Engineering, School of Agriculture, Shiraz University, Shiraz 71441-65186, Iran;2. Department of Mechanical Engineering of Biosystems, Faculty of Agriculture, Jahrom University, Jahrom 74135-111, Iran |
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| Abstract: | A radioimmunochemical method for the quantification of juvenile hormones from hemolymph and whole body extracts is described. Rabbit polyclonal antiserum developed against a JH III-bovine thyroglobulin conjugate displayed minimal cross-reactivity with juvenile hormone metabolites including juvenile hormone acids, juvenile hormone diols and analogs but substantial cross-reactivity between juvenile hormone homologs. Minimum sensitivity of the assay toward racemic juvenile hormone III was 65 pg. The degree and relative order of cross-reactivities for juvenile hormones I, II and III varied according to the identity of the radioligand used. A method for isolating juvenile hormones from whole body and hemolymph for radioimmunoassay was developed utilizing organic solvent extraction followed by thin-layer chromatography. Noninterfering dyes were used to bracket the position of the hormone on thin-layer chromatography plates. Hemolymph extracts known to contain no JH did not interfere with the assay when this procedure was employed. Radioimmunoassay analysis of hemolymph samples containing known amounts of JH and corrected for recovery yielded the expected results. Quantification of total juvenile hormone in whole body and hemolymph extracts of Manduca sexta was in good agreement with total mass of JH determined by a GC/MS method. |
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