The role of adipokinetic hormone in the control of vitellogenesis in locusts |
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| Institution: | 1. Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China;2. Key Lab of Holistic Integrative Enterology, Nanjing Medical University, Nanjing 210011, China;3. Department of Acupuncture and Oriental Medicine, Maryland University of Integrative Health, Laurel, MD, United States;4. Centre for Digestive Diseases, Five Dock, NSW 2046, Australia;1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China;2. Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China;3. International China-Belgium Joint Laboratory on Sustainable Crop Pest Control between Southwest University in China and Ghent University in Belgium, Chongqing 400715, China;4. Department of Plants and Crops, Ghent University, Ghent 9000, Belgium;1. Clermont Université, Université Blaise Pascal, Laboratoire “Microorganismes: Génome et Environnement”, BP 10448, F-63000 Clermont-Ferrand, France;2. CNRS, UMR 6023, LMGE, F-63171 Aubière, France;3. Clermont Université, Université Blaise Pascal, Institut Pascal, TSA 60206, CS 60026, 63178 Aubière, France;4. CNRS, UMR 6602, IP, F-63171 Aubière, France;1. Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 06, Bratislava, Slovakia;2. Institute of Molecular Physiology and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, 840 05, Bratislava, Slovakia |
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| Abstract: | Vitellogenesis in locusts is synchronized with the cyclic maturation of oocytes. Vitellogenesis by excised fat body of gravid females is differentially inhibited 80–90% when locust adipokinetic hormone I (AKH-I) is added to the incubation media. Hemolymph methanolic extracts completely inhibit fat body protein synthesis in vitro when the donor females are at the end of the ovarian cycle (6 mm stage), but not when taken from earlier stages. Hemolymph methanolic extracts from vitellogenic females at the 6 mm stage are separated by HPLC into three distinct inhibitors of protein synthesis, one of which is AKH-I. AKH-RIA of hemolymph during the first ovarian cycle reveals no AKH-I during active vitellogenesis, but a marked increase to about 5 ng per female at the end of egg maturation. A development of responsiveness to AKH-I is evident in female fat body as vitellogenesis proceeds. AKH-I is involved in the negative control of vitellogenesis. |
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