Isolation,identification and synthesis of locustamyotropin II,an additional neuropeptide of Locusta migratoria: Member of the cephalomyotropic peptide family |
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| Affiliation: | 1. Zoological Institute, Katholieke Universiteit Leuven, Naamsestraat 59, 3000 Leuven, Belgium;2. Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, F and B Road, College Station, TX 77841 USA;3. Laboratories for Invertebrate Neuroendocrine Research, Department of Entomology, Texas A&M University, College Station, TX 77843, U.S.A.;1. College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China;2. State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, 266580, China;3. Binzhou Key Laboratory of Material Chemistry, Department of Chemical Engineering and Safety, Binzhou University, Binzhou, 256603, China;1. Faculty of Life Sciences, Food Science, Hamburg University of Applied Sciences, Ulmenliet 20, 21033 Hamburg, Germany;2. Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Hermann-Weigmann-Str 1, 24103 Kiel, Germany;3. University of Hamburg, Hamburg School of Food Science, Grindelallee 117, 20146 Hamburg, Germany;1. UMR 1331 Toxalim, INRA, INP, UPS, Team 9 “Prevention, Promotion of Carcinogenesis by Food”, BP 93173, 180 chemin de Tournefeuille, 31027 Toulouse CEDEX, France;2. UMR 1331 Toxalim, INRA, INP, UPS, Axiom Platform, BP 93173, 180 chemin de Tournefeuille, 31027 Toulouse CEDEX, France;1. DPNC, University of Geneva, Quai Ansermet 24, CH-1205 Geneva, Switzerland;2. Physik Institut, University of Zurich, CH-8057 Zurich, Switzerland;3. ITPP, EPFL, CH-1015 Lausanne, Switzerland |
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| Abstract: | An eight residue neuropeptide (Glu-Gly-Asp-Phe-Thr-Pro-Arg-Leu-NH2) has been isolated from an extract of 9000 brain corpora cardiaca-corpora allata-suboesophageal ganglion complexes of Locusta migratoria. Biological activity was monitored during HPLC purification by observing the myotropic effect of column fractions on the isolated hindgut of Leucophaea maderae. The peptide designated as locustamyotropin II, or Lom-MT II according to Raina and Gäde (Insect Biochem.18, 785–787, 1988), has a Phe-X-Pro-Arg-Leu-NH2 carboxyl-terminal in common with the previously identified locustamyotropin I. Locustamyotropin II is also related to leucopyrokinin (Lem-PK), a blocked myotropic neuropeptide isolated from cockroach heads. Both peptides have identical carboxyterminal pentapeptide sequences. The constituent amino acids of this C-terminal are important for biological activity on the Leucophaea hindgut. Lom-MT II differs from Lem-PK in the first three aminoterminal residues. In contrast to Lem-PK and like Lom-MT I, the novel locust peptide is not N-terminally blocked. Lom-MT II has a stimulatory effect on the motility of the oviduct of Locusta but not on the hindgut. |
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