Molecular genetic manipulation of the red flour beetle: Genome organization and cloning of a ribosomal protein gene |
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| Institution: | 1. Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, U.S.A.;2. Department of Entomology, Waters Hall, Kansas State University, Manhattan, KS 66506, U.S.A.;1. Dipartimento di Chimica, Università degli Studi di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy;2. Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland;3. Istituto di Endocrinologia e Oncologia Sperimentale, CNR, 80138 Napoli, Italy;4. Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Napoli, Italy;5. Chemistry Department, Central University of Rajasthan, Kishangarh, Rajasthan, India;6. Department of Chemistry, University of North Texas, 1155, Union Circle, 305070 Denton, TX, United States;7. Faculty of Mathematics and Natural Sciences, School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego 1/3, 01-815 Warsaw, Poland;1. Departamento de Ciencias del Ambiente, Santiago, Chile;2. Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Av. L. B. O’Higgins 3363, Santiago, Chile;3. Departamento de Ciencias y Recursos Naturales, Facultad de Ciencias, Universidad de Magallanes, Av. Bulnes 1465, Punta Arenas, Chile;1. Institute of Biomechanics, Graz University of Technology, Stremayrgasse 16-II, 8010 Graz, Austria;2. Norwegian University of Science and Technology (NTNU), Faculty of Engineering Science and Technology, 7491 Trondheim, Norway;3. School of Mathematics and Statistics, University of Glasgow, University Place, Glasgow G12 8SQ, Scotland, UK;1. Central Laboratory, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;2. Department of Biochemistry, Indira Gandhi National Open University, New Delhi, India;3. Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India;4. Central Laboratory, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia |
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| Abstract: | We have assessed the potential for molecular genetic analysis of the red flour beetle, Tribolium castaneum, to augment the high resolution genetic analysis demonstrated for this organism by Beeman et al. (Dev. Biol.33, 196–209, 1989). The genome size, 0.21 pg, the long period interspersion pattern of repetitive elements and a low methyl-cytosine content indicate molecular studies should be straight-forward. We constructed a genomic library and isolated clones containing RPS14, a small, highly conserved, near single copy ribosomal protein gene. We found three RPS14 clones in a screen of four genome equivalents, suggesting our library is representative of the genome. We also demonstrated the use of an RFLP, identified by the RPS14 clone, in genetic mapping. |
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