Adipokinetic hormone causes formation of a low density lipophorin in the house cricket,Acheta domesticus |
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| Institution: | 1. School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, China;2. School of Medical Devices, Shenyang Pharmaceutical University, China;1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. National Marine Fisheries Service, Woods Hole, MA 02543, USA;2. National Marine Fisheries Service, Northeast Fisheries Science Center, Narragansett, RI 02882, USA;1. Laboratory of Malaria and Vector Research, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rockville, MD, 20852, USA;2. Laboratório de Bioquímica de Resposta ao Estresse, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil;3. Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China;4. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA;5. Structural Biology Section, Research Technologies Branch (RTB) National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, MD, 20892, USA |
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| Abstract: | In the house cricket, Acheta domesticus, injection of adipokinetic hormone induces the formation of a low density lipophorin via uptake of diacyglycerol from the fat body. Cricket low density lipophorin contains apolipophorin-III, which was purified and partially characterized. Cricket apolipophorin-III has a molecular weight of about 18,000, is not glycosylated, and has an isoelectric point of 4.8. Its amino acid composition is more similar to apolipophorin-III from Locusta migratoria than to that from Manduca sexta. The amino terminal sequence of cricket apolipophorin-III shows only limited homology to the amino terminal sequences of apolipophorin-III from L. migratoria and M. sexta. |
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