人隐静脉血管内皮细胞的分离、培养及鉴定

利用冠脉搭桥术后遗弃的隐静脉段获取内皮细胞，采用消化酶消化收集内皮细胞，扩增、冻存、复苏，在体外建立内皮细胞系。此方法简便易行，能在体外获得大量生物学特性保持良好的内皮细胞，为临床血管内皮化研究提供新的细胞来源。

In Vitro Culture of Human Saphenous Vein Endothelial Cells

The human saphenous vein endothelial cells were successfully dissociated and cultured by using the human saphenous vein segments leftover from cardiac bypass surgery. Endothelial cells were received, proliferated, frozen, and resuscitated by using digestive enzyme, The content of living cells produced using this method is very high and the passage cells were similar to the primary culture in shape and on the growth rate. It was also shown that endothelial cells line in vitro can be established simply and timely using this method.