Extracellular catalase activity protects cysteine cathepsins from inactivation by hydrogen peroxide |
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Authors: | Hervé-Grépinet Virginie Veillard Florian Godat Emmanuel Heuzé-Vourc'h Nathalie Lecaille Fabien Lalmanach Gilles |
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Affiliation: | INSERM U 618, Protéases et Vectorisation Pulmonaires, Equipe Protéases et Pathologies Pulmonaires, Tours F-37000, France. |
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Abstract: | The resistance of secreted cysteine cathepsins to peroxide inactivation was evaluated using as model THP-1 cells. Differentiated cells released mostly cathepsin B, but also cathepsins H, K, and L, with a maximum of endopeptidase activity at day 6. Addition of non-cytotoxic concentrations of H(2)O(2) did not affect mRNA expression levels and activity of cathepsins, while the catalase activity remained also unchanged, consistently with RT-PCR analysis. Conversely inhibition of extracellular catalase led to a striking inactivation of secreted cysteine cathepsins by H(2)O(2). This report suggests that catalase may participate in the protection of extracellular cysteine proteases against peroxidation. |
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Keywords: | AMC, 7-amino-4-methyl coumarin 3-AT, 3-amino-l, 2, 4-triazole BALF, bronchoalveolar lavage fluid BCA, bicinchoninic acid CA-074, N-( smallcaps" >l-3-trans-propylcarbamoyl oxirane-2-carbonyl)- smallcaps" >l-isoleucyl- smallcaps" >l-proline CP, cysteine protease DTT, smallcaps" >dl-dithiothreitol E-64, smallcaps" >l-3-carboxy-trans-2.3-epoxypropionyl-leucylamido-(4-guanidino) butane ECM, extra cellular matrix FBS, fetal bovine serum GM-CSF, granulocyte-macrophage colony-stimulating factor MDM, monocyte-derived macrophage MMTS, methylmethanethiosulfonate PMA, phorbol myristate acetate PMSF, phenylmethylsulfonyl fluoride ROS, reactive oxygen species Z, benzyloxycarbonyl |
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