High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes |
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| Authors: | Silvia Andrea Camperi Mariano Grasselli Osvaldo Cascone |
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| Affiliation: | (1) Facultad de Farmacia y Bioquímica, Cátedra de Microbiología Industrial y Biotecnología, UBA (1113) Junín 956, Buenos Aires, Argentina. E-mail |
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| Abstract: | Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane. |
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| Keywords: | hollow-fibre membranes IMAC pectic enzymes purification |
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