Antifungal secondary metabolites from endophytic Verticillium sp. |
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Authors: | Fei You Ting Han Jing-zhong Wu Bao-kang Huang Lu-ping Qin |
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Institution: | aDepartment of Pharmacognosy, School of Pharmacy, Second Military Medical University, Guohe Road 325, Shanghai 200433, PR China |
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Abstract: | Endophytes were isolated from roots of wild Rehmannia glutinosa to screen the strains with antifungal metabolites. A strain identified as Verticillium sp. was selected for chemical and biological investigations because of the strong antifungal activity of the crude extract against Pyricularia oryzae P-2b. Chemical investigations of culture broth afforded three compounds: 2,6-dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one, massariphenone and ergosterol peroxide. The metabolites were isolated by silica gel and Sephadex LH-20, 2,6-dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one was reported for the first time and the chemical structure was established following the analysis of NMR, UV, IR, MS data. 2,6-Dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one and ergosterol peroxide displayed clear inhibition of the growth of three pathogens as well as Verticillium sp. |
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Keywords: | Endophyte Antibiotic Inhibition Metabolite Verticillium sp |
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