贵阳地区蝉花微生态系统中细菌种群结构与功能

【目的】探索自然生态下蝉花内菌核、菌膜和环境细菌群落结构、功能及其相互关系。【方法】对细菌16S rRNA扩增片段进行高通量测序,分析贵阳市大将山和贵阳森林公园的蝉花内菌核、菌膜及其生境土壤的细菌群落组成、多样性及潜在功能。【结果】蝉花内菌核样本共检测到细菌562个属,菌膜样本521个属,菌际土样本578个属。两地的各组样本细菌群落结构相似,内菌核样本中假单胞菌属Pseudomonas、沙雷氏菌属Serratia占优势地位;菌膜样本中假单胞菌属和氨基杆菌属Aminobacter占优势;土壤样本以未分类的酸杆菌纲Acidobacteria和黄杆菌科Xanthobacteraceae为优势属。Venn图分析显示,菌际土样本包括了菌膜样本的大多数属,内菌核样本拥有较多的特有属,如沃尔巴克氏体属Wolbachia和立克次氏体Rickettsia等。PICRUSt功能预测结果显示,共计24个基因功能家族,主要与物质能量的代谢运输、细胞的行为发生及调控等功能相关。【结论】蝉花及其微生境中细菌具有丰富多样性,它们的潜在功能可能与营养物质的新陈代谢有关,对蝉花的个体生长发育有重要作用。研究结果对蝉花虫...

Bacterial community structure and function of Cordyceps cicadae microecosystem in Guiyang

[Objective] To explore the structure, function and relationship of bacterial community in sclerotia, cortices and habitat soil of Cordyceps cicadae under the natural ecology. [Methods] High-throughput sequencing of bacterial 16S rRNA amplification fragments was performed to analyze the composition, diversity and potential functions of bacterial community in sclerotia, cortices and habitat soil of Cordyceps cicadae in Mount Dajiang and Guiyang Forest Park. [Results] A total of 562 bacterial genera were detected in the sclerotia samples, 521 genera were detected in the cortices samples, and 578 genera were detected in the hyphosphere soil samples. The bacterial community structure of each group of samples from the two places was similar. In the sclerotia samples, Pseudomonas and Sphingobacterium were the dominant populations. Pseudomonas and Aminobacter were the dominant populations in the cortices samples. The norank_c_Acidobacteria and norank_f_Xanthobacteraceae were the dominant genera in the soil samples. Venn analysis showed that the hyphosphere soil samples included most genera of the cortices samples, and the sclerotia samples had more endemic genera, such as Wolbachia and Rickettsia. Furthermore, the PICRUSt function prediction results showed that there are a total of 24 gene function families, which are mainly related to the metabolism and transportation of material and energy, as well as the occurrence and regulation of cell behavior. [Conclusion] Bacteria in the Cordyceps cicadae and its microhabitat had rich diversity. Their potential function may be related to the metabolism of nutrients and played important roles in the individual growth and development of the Cordyceps cicadae. The results have reference value for supplementing the ecological information data of Cordyceps cicadae and imitateing wild cultivation.