怀牛膝细胞悬浮培养条件的优化

为进一步优化怀牛膝(Achyranthes bidentata)细胞悬浮培养条件,对接种量、继代周期、pH、光照及Cu2+等多种影响因子的作用效果进行了研究,以提高怀牛膝细胞生长量及牛膝多糖含量。结果显示,接种量50 g·L^–1、继代周期14天,pH5–6和光照培养可以使细胞保持良好的生长状态及多糖合成能力;添加50μmol·L^–1 Cu^2+,细胞的干重最大,可达44.63 g·L^–1,多糖含量也最高,为4.02 mg·g^–1。

Optimization of Cell Suspension Culture Conditions of Achyranthes bidentata

To optimize the suspension culture conditions of Achyranthes bidentata cells, effect of different factors like inoculum concentrations, subculture cycles, pH, light and Cu ²⁺ on cell growth and polysaccharides contents was studied. The results showed that, cells grew well with a good ability for polysaccharides synthesis under 50 g·L ^-1 inoculum concentration, 14 d subculture cycle, pH5-6 and light. In the presence of 50 μmol·L ^-1 Cu ²⁺, the cell dry weight could be the maximum with 44.63 g·L ^-1, and polysaccharides contents reached the peak at 4.02 mg·g ^-1.