| 植物蛋白SUMO化修饰检测方法 |
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| 引用本文： | 曲高平,金京波.植物蛋白SUMO化修饰检测方法[J].植物学报,2020,55(1):83-89. |
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| 作者姓名： | 曲高平 金京波 |
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| 作者单位： | 中国科学院植物研究所, 植物分子生理学重点实验室, 北京 100093 |
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| 基金项目： | 国际自然科学基金(31670186) |
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| 摘 要： | SUMO化是一种重要的蛋白质翻译后修饰,对植物正常生长发育不可或缺。到目前为止已筛选到上千个可能的SUMO底物,但由于SUMO化修饰水平普遍很低,其生物学功能研究相对较少。该文详细描述了检测蛋白SUMO化修饰的常用方法,包括体外和体内SUMO化实验,以及SUMO化修饰位点的检测方法,旨在为深入研究植物蛋白SUMO化修饰提供技术支持。
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| 关 键 词： | 拟南芥 SUMO SUMO化修饰 检测方法 |
| 收稿时间： | 2019-10-30 |
Detection of SUMOylation in Plants |
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| Gaoping Qu,Jingbo Jin.Detection of SUMOylation in Plants[J].Bulletin of Botany,2020,55(1):83-89. |
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| Authors: | Gaoping Qu Jingbo Jin |
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| Institution: | Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
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| Abstract: | SUMOylation, a post-translational modification, is essential for plant normal growth and development. To date, thousands of possible SUMO substrates have been identified, but due to the relatively low SUMOylation level, biological significance of the SUMOylation remains largely unknown. Here, we summarized the SUMOylation detection methods, including in vitro and in vivo SUMOylation assays, which help to understand the functions of SUMOylation in plants. |
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| Keywords: | Arabidopsis SUMO SUMOylation detection methods |
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