昆虫病原线虫的耐寒性

昆虫病原线虫可开发成生物农药，广泛应用于多种地下及钻蛀害虫的安全防治。但昆虫病原线虫货架期较短，对寒冷等极端环境的耐受性较差，影响了其在生物防治方面的商业开发。本文介绍了寒区的昆虫病原线虫资源，总结了昆虫病原线虫耐寒性的测定方法及增强方法、耐寒性差异的研究进展，并对其耐寒的生理生化机制及分子机理进行了综述。研究昆虫病原线虫的耐寒性，对于解释种群动态，指导昆虫病原线虫的低温保存，以及拓展其在生物防治方面的应用具有重要意义。

Cold tolerance of entomopathogenic nematodes

Entomopathogenic nematodes (EPNs) are biocontrol agents with high efficacy against insect pests and have been widely used to control different kinds of subterranean and boring pests. The short shelf life and the week tolerance to extreme temperatures in the environment hindered the large-scale application of these beneficial nematodes in biological control. In the present paper, EPN resources in cold regions were summarized. The methods to evaluate and increase the cold tolerance of EPNs, and the difference in cold tolerance among different species and strains of EPNs are introduced. The research progress in physiological, chemical and molecular mechanism of cold tolerance is also reviewed. Research in cold tolerance of EPNs is important as it will help to explain the population dynamics and provide basis for the preservation of EPN germplasm resources, so as to explore the commercial use of EPNs.