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| 包涵体蛋白的层析复性技术研究进展 | |
| 引用本文： | 高飞,范清林,邹文艺,宋礼华.包涵体蛋白的层析复性技术研究进展[J].生物技术通报,2006(2):56-59,71. |
| 作者姓名： | 高飞 范清林 邹文艺 宋礼华 |
| 作者单位： | 1. 安徽大学生命科学院,合肥,230039 2. 安徽安科生物工程股份有限公司,合肥,230088 3. 安徽省生物研究所,合肥,230088 |
| 摘 要： | 包涵体蛋白的复性是生物工程下游技术中的一个重要难题。层析法用于蛋白质复性是一种较新的、适用于大多数蛋白的方法。其原理是将层析技术应用于蛋白质复性和纯化,使变性蛋白质在层析柱上重折叠为正确的空间构象,在洗脱的同时实现部分纯化。本文详细介绍了蛋白质在5种层析柱上的复性方法、原理、应用及研究的新进展,为层析法对蛋白质复性的进一步应用提供依据。 |
| 关 键 词： | 包涵体 蛋白质复性 层析复性 重折叠 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2005-12-21 |
Progress in Renaturation of Inclusion Body Proteinsby Chromatographic Procedures |
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| Gao Fei,Fan Qinglin,Zou Wenyi,Song Lihua.Progress in Renaturation of Inclusion Body Proteinsby Chromatographic Procedures[J].Biotechnology Bulletin,2006(2):56-59,71. | |
| Authors: | Gao Fei Fan Qinglin Zou Wenyi Song Lihua |
| Institution: | 1 School of life science, Anhui University, Hefei 230039, China; 2 Anke biotechnology Co. , LTD, Hefei 230088, China ;3 Biology Institute of Anhui Province, Hefei 230088 |
| Abstract: | Inclusion body protein renaturation is a important procedure in the biotechnology.Protein refolding by chromatography is a new and universal technology.The unfolded protein correctly refolded to the native conformation through the column,and was partly purified during the elution process.The method,principle,recent development for renaturation of proteins by five kinds of chromatography,hydrophobic interaction chromatography,ion exchange chromatography,size exclusion chromatography,affinity chromatography and expanded bed adsorption chromatography were introduced in details.The new method may be used for researching the renaturation of proteins in preparative,even in productive scales in industry. |
| Keywords: | Inclusion body Protein renaturation Chromatography renaturation Refolding |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |