Change of concanavalin A induced agglutinability during preimplantation mouse development |
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Authors: | J Rowinski D Solter H Koprowski |
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Institution: | The Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104 USA |
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Abstract: | Mouse embryos during early cleavage (zygote to eight-cell stage) were agglutinable with a low concentration (10 μg/ml) of concanavalin A (ConA). This agglutinability was reduced during the first mitotic division. Morulae were agglutinable with a slightly higher concentration (100 μg/ml), whereas blastocysts were not agglutinable even with ConA at a concentration of 5000 μg/ml; however, isolated inner cell masses agglutinated readily at 10 μg/ml of ConA. Embryos grown in vitro behaved as did those isolated directly from the genital tract. Treatment with proteolytic enzymes did not induce agglutinability of mouse blastocyst. The change in agglutinability of trophoblastic cells reflects dramatic changes in the cell surface. |
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Keywords: | To whom reprint requests should be addressed (36th str at Spruce) |
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