Reactivity of elementary and reticulate bodies of Chlamydia trachomatis LGV2 with monoclonal antibodies specific for the major outer membrane protein |
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Authors: | R. Cevenini M. Donati V. Sambri F. Rumpianesi M. La Placa |
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Affiliation: | Institute of Microbiology, University of Bologna, Ospedale S. Orsola, Bologna, Italy |
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Abstract: | The reactivity of the major outer membrane protein (MOMP) of Chlamydia trachomatis (LGV2 serotype) with 15 monoclonal antibodies was studied during the course of developmental cycle by immunoblotting and immunofluorescence. The monoclonal antibodies reacted in immunoblots with the MOMP of both elementary bodies (EBs) and reticulate bodies (RBs). Using an immunofluorescence test with LGV2-infected cell cultures, the 15 monoclonal antibodies could be divided into 5 groups, according to the time of appearance of their reactivity with the cell culture. |
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Keywords: | Chlamydia trachomatis Elementary body Reticulate body Outer membrane protein (Immunoblotting Monoclonal antibody) |
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