Human thymocyte maturation in vitro: a flow cytometric analysis |
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| Authors: | M L Blue J F Daley H Levine S F Schlossman |
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| Affiliation: | 1. Department of Microbiology and Immunology, Western University, London, ON, Canada;2. Department of Oncology, Western University, London, ON, Canada;3. Department of Otolaryngology, Western University, London, ON, Canada;4. London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada;1. Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;2. Institute of Hypertension, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China;3. Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China |
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| Abstract: | Using an in vitro culture system, light scatter analyses, and two-color flow cytometry, we provide evidence that the interleukin-2 (IL-2) and transferrin receptors can be induced within 48 hr on nonproliferating immature thymocytes. The thymocytes (greater than 35%) that expressed the transferrin and IL-2 receptors demonstrated nuclear activation as measured by log 90 degrees light scatter analysis. Increases in antigen-receptor-associated T3-antigen expression followed transferrin and IL-2-receptor induction and occurred on maximally activated T4+T8+ thymocytes on Day 3 of culture. Maximal T3 expression did not occur until Days 5-7 and paralleled loss of T4, T8 coexpression, suggesting an association between a mature T3-Ti antigen receptor complex and a mature T4, T8 phenotype. |
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