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Flare-up of delayed-type hypersensitivity initially induced by murine cloned helper T cells
Authors:I S Klasen  T H van der Kwast  I G Donselaar  R M Ladestein  R Benner
Affiliation:1. Department of Cell Biology, Immunology and Genetics, Erasmus University, P.O. Box 1738, 3000DR Rotterdam, The Netherlands;1. Department of Pathology, Erasmus University, P.O. Box 1738, 3000DR Rotterdam, The Netherlands;1. Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA;2. Retinal Neurophysiology Section, National Eye Institute, NIH, Bethesda, MD, USA;3. National Eye Institute, National Institutes of Health, Bethesda, MD, USA;4. Department of Surgery, University of Colorado School of Medicine, CU Anschutz Medical Campus, Aurora, CO 80045, USA;5. Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA;1. Department of Dermatology, Temple University Hospital, Philadelphia, Pennsylvania;2. Baylor Scott & White Health System, Dallas, Texas;3. Bare Dermatology, Dallas, Texas;4. Vivida Dermatology, Las Vegas, Nevada;5. Castle Biosciences, Inc, Friendswood, Texas;6. Department of Dermatology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;1. Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, Stanford University School of Medicine, Stanford, Calif;2. Department of Dermatology, Stanford University, Stanford, Calif;3. Department of Pathology, Stanford University, Stanford, Calif;4. Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, Calif
Abstract:Delayed-type hypersensitivity (DTH) reactions were induced in mice by cloned helper T cells directed against methylated bovine serum albumin (mBSA). The DTH reactions were induced either by local injection of the helper T cells together with the antigen in the hind feet or by intravenous (iv) administration of the cloned T cells and local injection of the antigen. Local or systemic (oral or iv) administration of mBSA after waning of the DTH induced by the cloned helper T cells caused a flare-up reaction. This indicates that functional helper T cells persist at the inflammation site. The inflammations were quantified in a foot swelling assay and were examined histologically. The inflammation measured in the flare-up reaction was generally lower than in the acute reaction. Histologically the acute inflammation showed edema and a large proportion of granulocytes, whereas the flare-up reaction appeared more histiocytic and showed less edema.
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