小麦TaMAPK2基因的克隆及表达分析

该研究从小麦中克隆了1个MAPK基因TaMAPK2。序列分析表明,TaMAPK2基因的ORF为1 110bp,编码369个氨基酸。序列比对分析表明,该基因所编码的蛋白与粗山羊草、水稻、谷子等MAPK蛋白具有较高的一致性,分别为99%、94%、94%。进化树分析表明,TaMAPK2与水稻OsMAPK2的亲缘关系最近。实时荧光定量PCR分析表明,该基因的表达显著受渗透胁迫、低温胁迫、高盐胁迫、乙烯和双氧水诱导,受ABA抑制。研究表明,TaMAPK2可能参与非生物逆境胁迫及相关信号分子应答。

Cloning and Expression Analysis of TaMAPK2 Gene in Wheat

MAPK protein kinase plays an important role in the response of abiotic stresses.In the present study,we isolated a MAPK gene designated TaMAPK2 from wheat.TaMAPK2 ORF is 1 110 bp,which encodes 369 amino acids.Comparison of amino acid sequences indicated that TaMAPK2 shared high similarity with MAPKs from other species,such as Aegilops tauschii (99%),Oryza sativa (94%) and Setaria italica (94%).Phylogenetic analysis showed that TaMAPK2 is closely related with OsMAPK2.Real-time quantitative polymerase chain reaction (QPCR) assay revealed that TaMAPK2 expression is induced after osmotic stress,low temperature,salt stress,ethylene and H₂O₂ treatments and inhibited after ABA treatment.These results suggested that TaMAPK2 participates in the response of abiotic stresses and related signaling molecules.